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elastin-congo red (ecr  (Millipore)


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    Structured Review

    Millipore elastin-congo red (ecr
    Elastin Congo Red (Ecr, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elastin+congo+red+(ecr)+assay/elastin+congo+red/pm39153575-145-22-25
    Average 90 stars, based on 1 article reviews
    elastin-congo red (ecr - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Quorum sensing systems, related virulence factors, and biofilm formation in Pseudomonas aeruginosa isolated from fish.
    Article Snippet: In this study, two quorum sensing (QS) system genes, las and rhI; N-3-oxo-dodecanoyl homoserine lactone (AHL; 3-O-C12HSL); and QS-related virulence factors and correlation between them were assessed in 30 fish origin P. aeruginosa isolates.. The detection of two QS system of the isolates, and eight gene regions consisting of four intact (lasI/R, rhlI/R) and four internal (lasI/R, rhlI/R) genes were tested by PCR assay.. According to findings, las and rhI QS system genes were detected in 27 and 30 isolates, respectively, while 3-O-C12-HSL was determined in 13 isolates.

    Article Title: The anti-quorum sensing and biofilm inhibitory potential of Piper betle L. leaf extract and prediction of the roles of the potent phytocompounds.
    Article Snippet: The leaves of Piper betle L., known as betel leaf, have immense medicinal properties.. It possesses potent antimicrobial efficacies and can be a valuable tool to combat drug-resistant microorganisms.. Quorum sensing (QS) inhibition is one of the best strategies to combat drug resistance.

    Article Title: Antimicrobial resistance, virulence factors, and genotypes of Pseudomonas aeruginosa clinical isolates from Gorgan, northern Iran.
    Article Snippet: Pseudomonasaeruginosa is an important nosocomial pathogen with a capacity of resistance to multiple antibiotics and production of various extracellular and cell-associated virulence factors that clearly contribute to its pathogenicity.. The objective of this study was to investigate the antibiotic susceptibility, virulence factors, and clonal relationship among clinical isolates of P. aeruginosa.. Different clinical specimens from hospitalized patients were investigated for P. aeruginosa.

    Article Title: Quercetin: a promising virulence inhibitor of Pseudomonas aeruginosa LasB in vitro
    Article Snippet: Elastin-Congo red (ECR) (Sigma-Aldrich, SHBL9368) was used to determine the LasB elastase activity as previously published (Yang et al. ).

    Article Title: The Transcriptional Regulator CzcR Modulates Antibiotic Resistance and Quorum Sensing in Pseudomonas aeruginosa
    Article Snippet: The LasB elastase activity of bacterial suspensions was determined with the Elastin-Congo red (ECR; Sigma, St. Louis, MO) assay , with minor modifications.

    Activity Assay:

    Article Title: Quinic acid: a potential antibiofilm agent against clinical resistant Pseudomonas aeruginosa
    Article Snippet: .. For determining elastase activity, PA with different concentrations of LJF (1/4MIC, 1/8MIC, 1/16MIC) was grown at 37 °C for 18 h and then sterile supernatant (100 μL) was mixed with elastin Congo red (ECR, Sigma-Aldrich) buffer (900 μL, 1 mM CaCl 2 , 100 mM Tris, pH 7.5) containing 20 mg of ECR and incubated at 37 °C for 5 h. After incubation, the absorbance of the supernatant was determined at 495 nm. ..

    Article Title: Anti-quorum sensing activity in Pseudomonas aeruginos a PA01 of benzimidazolium salts: electronic, spectral and structural investigations as theoretical approach.
    Article Snippet: Quorum sensing (QS) is a system used in the expression of virulence factors by many pathogenic bacteria, and blockage of the system is seen as a new and effective strategy in combating with resistant bacteria.. The inhibition effects of two benzimidazolium salts, namely 1-(2-methylbenzonitrile)-3benzylbenzimidazolium bromide (2) and 1-(N-methylphthalimide)-3-(4-methylbenzyl)benzimidazolium bromide (3), on quorum sensing-related virulence factors such as pyocyanin, elastase, biofilm formation and swarming motility, which is an opportunistic pathogen Pseudomonas aeruginosa PA01, were investigated in this study.. The results show that the compound 3 has a significant inhibition on biofilm formation with 94%.

    Article Title: Phytochemical composition and antimicrobial, and anti-quorum sensing activities of Punica granatum L. methanolic extract
    Article Snippet: .. Elastin Congo red (ECR; Sigma, St. Louis, USA) assay was used to measure elastase activity. .. PAO1 was grown in Luria-Bertani broth (LB; Sigma Aldrich, USA) at 37°C and the different concentrations of methanolic extracts (0.5; 1; 2.5; 5 and10 mg/ml).

    Sterility:

    Article Title: Quinic acid: a potential antibiofilm agent against clinical resistant Pseudomonas aeruginosa
    Article Snippet: .. For determining elastase activity, PA with different concentrations of LJF (1/4MIC, 1/8MIC, 1/16MIC) was grown at 37 °C for 18 h and then sterile supernatant (100 μL) was mixed with elastin Congo red (ECR, Sigma-Aldrich) buffer (900 μL, 1 mM CaCl 2 , 100 mM Tris, pH 7.5) containing 20 mg of ECR and incubated at 37 °C for 5 h. After incubation, the absorbance of the supernatant was determined at 495 nm. ..

    Incubation:

    Article Title: Quinic acid: a potential antibiofilm agent against clinical resistant Pseudomonas aeruginosa
    Article Snippet: .. For determining elastase activity, PA with different concentrations of LJF (1/4MIC, 1/8MIC, 1/16MIC) was grown at 37 °C for 18 h and then sterile supernatant (100 μL) was mixed with elastin Congo red (ECR, Sigma-Aldrich) buffer (900 μL, 1 mM CaCl 2 , 100 mM Tris, pH 7.5) containing 20 mg of ECR and incubated at 37 °C for 5 h. After incubation, the absorbance of the supernatant was determined at 495 nm. ..

    Inhibition:

    Article Title: Anti-quorum sensing activity in Pseudomonas aeruginos a PA01 of benzimidazolium salts: electronic, spectral and structural investigations as theoretical approach.
    Article Snippet: Quorum sensing (QS) is a system used in the expression of virulence factors by many pathogenic bacteria, and blockage of the system is seen as a new and effective strategy in combating with resistant bacteria.. The inhibition effects of two benzimidazolium salts, namely 1-(2-methylbenzonitrile)-3benzylbenzimidazolium bromide (2) and 1-(N-methylphthalimide)-3-(4-methylbenzyl)benzimidazolium bromide (3), on quorum sensing-related virulence factors such as pyocyanin, elastase, biofilm formation and swarming motility, which is an opportunistic pathogen Pseudomonas aeruginosa PA01, were investigated in this study.. The results show that the compound 3 has a significant inhibition on biofilm formation with 94%.



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